[ effect of two non- cloned intermediate Iranian and foreign infectious bursal disease vaccines on broiler chicks performance]

Background: comparison between Infectious Bursal Disease vaccines

Objectives: comparison between foreign and Iranian intermediate uncloned Gumboro vaccines on feed conversion ratio

Methods: three hundred and thirty day-old chicks were purchased and for determination of vaccination time by Deventer formula, thirty chicks were randomly bled and the remaining chicks divided into 3 equal groups, each group was divided into 4 equal subgroups with the same mean weight. On the basis of Elisa results and vaccine instructions, chicks of group 1 and 2 were vaccinated by uncloned intermediate Gumboro vaccine manufactured by Lohman Company, Germany and Razi Vaccine and Serum Research Institute, Iran subsequently at 16 and 23 days by drinking water. One group was kept as unvaccinated control group. Mean feed intake, weight gains, feed conversion ratio and B:B ratio of 3 groups were determined at 16, 23 and 42 days. At the end of experiment B:B ratio was measured in 3 groups

Results: this study showed that foreign and Iranian intermediate uncloned Gumboro vaccines had negative effect on mean weight gains and both vaccines atrophied bursa of Fabricius, but had no effect on mean feed intake and feed conversion ratio

Conclusions: this study showed that foreign and Iranian intermediate uncloned Gumboro vaccines had no effect on feed conversion ratio

Characterization of Eimeria species in commercial broilers by PCR based on ITS1 regions of rDNA

Coccidiosis is an intestinal disease of chickens caused by various species of protozoan parasites within the genus Eimeria. Diagnosis and genetic characterization of different species of Eimeria are central to the prevention, surveillance, and control of coccidiosis. The aim of this study was to detect different chicken Eimeria species from several areas in Khuzestan, south-west Iran. From February to September 2008, PCR assay as well as parasitological examinations was applied for the identification of field isolates of Eimeria parasites around Ahvaz, center of Khuzestan, southwest Iran. Data were analyzed bythe Kappa statistic test. Eimeria maxima, E. necatrix, E. tenella, E. acervulina and E. mitis were detected in this study. The prevalence of Eimeria spp. was 31.5% [126 and 400] and E. tenella was the most prevalent species in Khuzestan. Based on the Kappa statistical test, a good correlation between the results of PCR and traditional biometrical methods was only observed for E. maxima. The present study is the first on the prevalence of Eimeria species in Khuzestan, based on the molecular findings. We believe that traditional methods are not sufficiently reliable for specific diagnosis of Eimeria species in chickens and PCR based amplification of DNA sequence of parasite, could resolve this problem

Isolation of Riemerella anatipestifer from Domestic Ducks in Rasht area

Investigation of the presence of R. anatipestife r in Rasht area [North of Iran]. 109 domestic ducks and 33 hen and rosters . Samples were collected from pasteurellosis suspected birds on the basis of clinical signs and gross lesions. Tissue samples from liver, lungs, air sacs, heart blood and synovial fluid of each suspected birds were cultured on blood agar according routine methods. The cultures were incubated under candle jar at 37 c for 24-72 hours. Isolated pasteurella spp. Was identified on the basis of colony and cell morphology and biochemical reactions. Isolates of Riemerella assigned to species according Rimller 1998. P. multocida was isolated from 46 [42.2ko[3]/41] out of 109 ducks, and from 12 [63.3ko%l] of 33 hen and rosters. R. anatipestifer was isolated from 13 [11.9ko[3]/41] ducks but nither from hens nor from rosters. Other species of pasteurella viz. P. gallinarum and P. hemolytica were not isolated. Antimicrobial sensitivity test of isolated Riemerella showed that the most sensitive antibiotics were flumoquine, trimetoprim with 92.3ko[3]/41 and the most resistance antibiotics was oxytetracycline [30.8ko[3]/41] and penicillin [23.Iko[3]/[41]. This investigation revealed that R. anatipestifer is present in domestic Ducks in this area, but not in domestichen and rosters